Marcelo E. Guerin

Ikerbasque Research Professor, Biocruces Bizkaia

Group Leader at Biocruces Bizkaia Health Research Institute.

Research line:

Structural Glycobiology.

Post-doctoral Fellow at the Structural Biochemistry Unit, Institut Pasteur, Paris, France (2003-2007) and Staff Research Scientist at the Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, USA (2007-2009).

Ph.D. in Biochemistry and Molecular Biology from the Leloir Institute, School of Sciences, University of Buenos Aires, Argentina (2002).

Publicaciones

2023

Article / SigH stress response mediates killing of Mycobacterium tuberculosis by activating nitronaphthofuran prodrugs via induction of Mrx2 expression

NUCLEIC ACIDS RESEARCH

Cioetto-Mazzabò, L.; Boldrin, F.; Beauvineau, C.; Speth, M.; Marina, A.; Namouchi, A.; Segafreddo, G.; Cimino, M.; Favre-Rochex, S.; Balasingham, S.; Trastoy, B.; Munier-Lehmann, H.; Griffiths, G.; Gicquel, B.; Guerin, M.E.; Manganelli, R.; Alonso-Rodríguez, N.

Article / Mechanism of high-mannose N-glycan breakdown and metabolism by Bifidobacterium longum

Nature Chemical Biology

Cordeiro, R.L.; Santos, C.R.; Domingues, M.N.; Lima, T.B.; Pirolla, R.A.S.; Morais, M.A.B.; Colombari, F.M.; Miyamoto, R.Y.; Persinoti, G.F.; Borges, A.C.; de Farias, M.A.; Stoffel, F.; Li, C.; Gozzo, F.C.; van Heel, M.; Guerin, M.E.; Sundberg, E.J.; Wang, L.X.; Portugal, R.V.; Giuseppe, P.O.; Murakami, M.T.

Article / Mechanism of antibody-specific deglycosylation and immune evasion by Streptococcal IgG-specific endoglycosidases

Nature Communications

Trastoy, B.; Du, J.J.; Cifuente, J.O.; Rudolph, L.; García-Alija, M.; Klontz, E.H.; Deredge, D.; Sultana, N.; Huynh, C.G.; Flowers, M.W.; Li, C.; Sastre, D.E.; Wang, L.X.; Corzana, F.; Mallagaray, A.; Sundberg, E.J.; Guerin, M.E.

Article / Turning universal O into rare Bombay type blood

Nature Communications

Anso, I.; Naegeli, A.; Cifuente, J.O.; Orrantia, A.; Andersson, E.; Zenarruzabeitia, O.; Moraleda-Montoya, A.; García-Alija, M.; Corzana, F.; Del Orbe, R.A.; Borrego, F.; Trastoy, B.; Sjögren, J.; Guerin, M.E.

2022

Article / Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE

Nature Communications

García-Alija, M.; Du, J.J.; Ordóñez, I.; Diz-Vallenilla, A.; Moraleda-Montoya, A.; Sultana, N.; Huynh, C.G.; Li, C.; Donahue, T.C.; Wang, L.X.; Trastoy, B.; Sundberg, E.J.; Guerin, M.E.

Review / Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases

Current Opinion in Structural Biology

Trastoy, B.; Du, J.J.; García-Alija, M.; Li, C.; Klontz, E.H.; Wang, L.X.; Sundberg, E.J.; Guerin, M.E.

2021

Article / Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA

Science Advances

Anso, I.; Basso, L.G.M.; Wang, L.; Marina, A.; Páez-Pérez, E.D.; Jäger, C.; Gavotto, F.; Tersa, M.; Perrone, S.; Xabier Contreras, F.; Prandi, J.; Gilleron, M.; Linster, C.L.; Corzana, F.; Lowary, T.L.; Trastoy, B.; Guerin, M.E.

Article / GH18 endo-β-N-acetylglucosaminidases use distinct mechanisms to process hybrid-type N-linked glycans

Journal of Biological Chemistry

Trastoy, B.; Du, J.J.; Li, C.; García-Alija, M.; Klontz, E.H.; Roberts, B.R.; Donahue, T.C.; Wang, L.X.; Sundberg, E.J.; Guerin, M.E.

Article / The phosphatidyl-myo-inositol dimannoside acyltransferase pata is essential for mycobacterium tuberculosis growth in vitro and in vivo

Journal of Bacteriology

Boldrin, F.; Anso, I.; Alebouyeh, S.; Sevilla, I.A.; Geijo, M.; Garrido, J.M.; Marina, A.; Mazzabò, L.C.; Segafreddo, G.; Guerin, M.E.; Manganelli, R.; Prados-Rosales, R.

2020

Article / Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila

Nature Communications

Trastoy, B.; Naegeli, A.; Anso, I.; Sjögren, J.; Guerin, M.E.

Article / Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides

Nature Communications

Trastoy, B.; Du, J.J.; Klontz, E.H.; Li, C.; Cifuente, J.O.; Wang, L.X.; Sundberg, E.J.; Guerin, M.E.

Article / The allosteric control mechanism of bacterial glycogen biosynthesis disclosed by cryoEM

Current Research in Structural Biology

Cifuente, J.O.; Comino, N.; D'Angelo, C.; Marina, A.; Gil-Carton, D.; Albesa-Jové, D.; Guerin, M.E.

Article / Dissecting the Structural and Chemical Determinants of the "open-To-Closed" Motion in the Mannosyltransferase PimA from Mycobacteria

Biochemistry

Rodrigo-Unzueta, A.; Ghirardello, M.; Urresti, S.; Delso, I.; Giganti, D.; Anso, I.; Trastoy, B.; Comino, N.; Tersa, M.; D'Angelo, C.; Cifuente, J.O.; Marina, A.; Liebau, J.; Mäler, L.; Chenal, A.; Albesa-Jové, D.; Merino, P.; Guerin, M.E.

Article / Unveiling the activation dynamics of a fold-switch bacterial glycosyltransferase by <sup>19</sup>F NMR

Journal of Biological Chemistry

Liebau, J.; Tersa, M.; Trastoy, B.; Patrick, J.; Rodrigo-Unzueta, A.; Corzana, F.; Sparrman, T.; Guerin, M.E.; Mäler, L.

Article / Membrane fluidity adjusts the insertion of the transacylase PlsX to regulate phospholipid biosynthesis in Gram-positive bacteria

Journal of Biological Chemistry

Sastre, D.E.; Basso, L.G.M.; Trastoy, B.; Cifuente, J.O.; Contreras, X.; Gueiros-Filho, F.; de Mendoza, D.; Navarro, M.V.A.S.; Guerin, M.E.

Review / Structural insights into the mechanisms and specificities of IgG-active endoglycosidases

Glycobiology

Du, J.J.; Klontz, E.H.; Guerin, M.E.; Trastoy, B.; Sundberg, E.J.

2019

Article / Regulation of Human Hsc70 ATPase and Chaperone Activities by Apg2: Role of the Acidic Subdomain

Journal of Molecular Biology

Cabrera, Y.; Dublang, L.; Fernández-Higuero, J.A.; Albesa-Jové, D.; Lucas, M.; Viguera, A.R.; Guerin, M.E.; Vilar, J.M.G.; Muga, A.; Moro, F.

Article / Structure-function relationships underlying the dual N-acetylmuramic and N-acetylglucosamine specificities of the bacterial peptidoglycan deacetylase PdaC

Journal of Biological Chemistry

Grifoll-Romero, L.; Sainz-Polo, M.A.; Albesa-Jové, D.; Guerin, M.E.; Biarnés, X.; Planas, A.

Article / Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2

ACS Central Science

Klontz, E.H.; Trastoy, B.; Deredge, D.; Fields, J.K.; Li, C.; Orwenyo, J.; Marina, A.; Beadenkopf, R.; Günther, S.; Flores, J.; Wintrode, P.L.; Wang, L.X.; Guerin, M.E.; Sundberg, E.J.

Article / 3-Phosphoglycerate Transhydrogenation Instead of Dehydrogenation Alleviates the Redox State Dependency of Yeast de Novo l -Serine Synthesis

Biochemistry

Paczia, N.; Becker-Kettern, J.; Conrotte, J.F.; Cifuente, J.O.; Guerin, M.E.; Linster, C.L.

Review / Structural basis of glycogen metabolism in bacteria

Biochemical Journal

Cifuente, J.O.; Comino, N.; Trastoy, B.; D’Angelo, C.; Guerin, M.E.

Review / The Mycobacterium tuberculosis capsule: A cell structure with key implications in pathogenesis

Biochemical Journal

Kalscheuer, R.; Palacios, A.; Anso, I.; Cifuente, J.; Anguita, J.; Jacobs, W.R.; Guerin, M.E.; Prados-Rosales, R.

2018

Article / Structural basis for the recognition of complex-Type N-glycans by Endoglycosidase S

Nature Communications

Trastoy, B.; Klontz, E.; Orwenyo, J.; Marina, A.; Wang, L.X.; Sundberg, E.J.; Guerin, M.E.

Article / Conformational entropy of a single peptide controlled under force governs protease recognition and catalysis

Proceedings of the National Academy of Sciences of the United States of America

Guerin, M.E.; Stirnemann, G.; Giganti, D.

Article / The Molecular Mechanism of Substrate Recognition and Catalysis of the Membrane Acyltransferase PatA from Mycobacteria

ACS Chemical Biology

Tersa, M.; Raich, L.; Albesa-Jové, D.; Trastoy, B.; Prandi, J.; Gilleron, M.; Rovira, C.; Guerin, M.E.

2017

Article / The antibacterial prodrug activator Rv2466c is a mycothiol-dependent reductase in the oxidative stress response of Mycobacterium tuberculosis

Journal of Biological Chemistry

Rosado, L.A.; Wahni, K.; Degiacomi, G.; Pedre, B.; Young, D.; De la Rubia, A.G.; Boldrin, F.; Martens, E.; Marcos-Pascual, L.; Sancho-Vaello, E.; Albesa-Jové, D.; Provvedi, R.; Martin, C.; Makarov, V.; Versées, W.; Verniest, G.; Guerin, M.E.; Mateos, L.M.; Manganelli, R.; Messens, J.

Article / Structural Snapshots and Loop Dynamics along the Catalytic Cycle of Glycosyltransferase GpgS

Structure

Albesa-Jové, D.; Romero-García, J.; Sancho-Vaello, E.; Contreras, F.X.; Rodrigo-Unzueta, A.; Comino, N.; Carreras-González, A.; Arrasate, P.; Urresti, S.; Biarnés, X.; Planas, A.; Guerin, M.E.

Article / Mechanistic insights into the allosteric regulation of bacterial ADP-glucose pyrophosphorylases

Journal of Biological Chemistry

Comino, N.; Cifuente, J.O.; Marina, A.; Orrantia, A.; Eguskiza, A.; Guerin, M.E.

Letter / Structural snapshots of α-1,3-galactosyltransferase with native substrates: insight into the catalytic mechanism of retaining glycosyltransferases.

Angewandte Chemie - International Edition

Albesa-Jové, D., Sainz-Polo, M.A., Marina, A., Guerin, M.E.

Review / Structural basis of phosphatidyl-myo-inositol mannosides biosynthesis in mycobacteria

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Sancho-Vaello, E.; Albesa-Jové, D.; Rodrigo-Unzueta, A.; Guerin, M.E.

2016

Article / Structural Basis of Glycogen Biosynthesis Regulation in Bacteria

Structure

Cifuente, J.O.; Comino, N.; Madariaga-Marcos, J.; López-Fernández, S.; García-Alija, M.; Agirre, J.; Albesa-Jové, D.; Guerin, M.E.

Article / Molecular basis of membrane association by the phosphatidylinositol mannosyltransferase PimA enzyme from mycobacteria

Journal of Biological Chemistry

Rodrigo-Unzueta, A.; Martínez, M.A.; Comino, N.; Alzari, P.M.; Chenal, A.; Guerin, M.E.

Article / Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA

Nature Communications

Albesa-Jové, D.; Svetlíková, Z.; Tersa, M.; Sancho-Vaello, E.; Carreras-González, A.; Bonnet, P.; Arrasate, P.; Eguskiza, A.; Angala, S.K.; Cifuente, J.O.; Korduláková, J.; Jackson, M.; Mikušová, K.; Guerin, M.E.

Review / The conformational plasticity of glycosyltransferases

Current Opinion in Structural Biology

Albesa-Jové, D.; Guerin, M.E.

2015

Article / The redox state regulates the conformation of Rv2466c to activate the antitubercular prodrug TP053

Journal of Biological Chemistry

Albesa-Jove, D.; Comino, N.; Tersa, M.; Mohorko, E.; Urresti, S.; Dainese, E.; Chiarelli, L.R.; Pasca, M.R.; Manganelli, R.; Makarov, V.; Riccardi, G.; Svergun, D.I.; Glockshuber, R.; Guerin, M.E.

Article / A Native Ternary Complex Trapped in a Crystal Reveals the Catalytic Mechanism of a Retaining Glycosyltransferase

Angewandte Chemie - International Edition

Albesa-Jové, D.; Mendoza, F.; Rodrigo-Unzueta, A.; Gomollõn-Bel, F.; Cifuente, J.O.; Urresti, S.; Comino, N.; Gõmez, H.; Romero-García, J.; Lluch, J.M.; Sancho-Vaello, E.; Biarnés, X.; Planas, A.; Merino, P.; Masgrau, L.; Guerin, M.E.

Article / Secondary structure reshuffling modulates glycosyltransferase function at the membrane

Nature Chemical Biology

Giganti, D.; Albesa-Jové, D.; Urresti, S.; Rodrigo-Unzueta, A.; Martínez, M.A.; Comino, N.; Barilone, N.; Bellinzoni, M.; Chenal, A.; Guerin, M.E.; Alzari, P.M.

2014

Article / Rv2466c mediates the activation of TP053 to kill replicating and non-replicating Mycobacterium tuberculosis

ACS Chemical Biology

Albesa-Jové, D.; Chiarelli, L.R.; Makarov, V.; Pasca, M.R.; Urresti, S.; Mori, G.; Salina, E.; Vocat, A.; Comino, N.; Mohorko, E.; Ryabova, S.; Pfieiffer, B.; Lopes Ribeiro, A.L.D.J.; Rodrigo-Unzueta, A.; Tersa, M.; Zanoni, G.; Buroni, S.; Altmann, K.H.; Hartkoorn, R.C.; Glockshuber, R.; Cole, S.T.; Riccardi, G.; Guerin, M.E.

Article / Structural basis of chitin oligosaccharide deacetylation

Angewandte Chemie - International Edition

Andrés, E.; Albesa-Jové, D.; Biarnés, X.; Moerschbacher, B.M.; Guerin, M.E.; Planas, A.

Article / The phosphatidyl-myo-inositol mannosyltransferase PimA is essential for Mycobacterium tuberculosis growth in vitro and in vivo

Journal of Bacteriology

Boldrin, F.; Ventura, M.; Degiacomi, G.; Ravishankar, S.; Sala, C.; Svetlikova, Z.; Ambady, A.; Dhar, N.; Kordulakova, J.; Zhang, M.; Serafini, A.; Vishwas, V.G.; Kolly, G.S.; Kumar, N.; Palu`, G.; Guerin, M.E.; Mikusova, K.; Cole, K.M.; Manganelli, R.

Review / Structure-function relationships of membrane-associated GT-B glycosyltransferases

Glycobiology

Albesa-Jové, D.; Giganti, D.; Jackson, M.; Alzari, P.M.; Guerin, M.E.

2013

Article / Conformational plasticity of the essential membrane-associated mannosyltransferase PimA from mycobacteria

Journal of Biological Chemistry

Giganti, D.; Alegre-Cebollada, J.; Urresti, S.; Albesa-Jové, D.; Rodrigo-Unzueta, A.; Comino, N.; Kachala, M.; López-Fernández, S.; Svergun, D.I.; Fernández, J.M.; Guerin, M.E.

Article / Structural Basis for Feed-Forward Transcriptional Regulation of Membrane Lipid Homeostasis in Staphylococcus aureus

PLoS Pathogens

Albanesi, D.; Reh, G.; Guerin, M.E.; Schaeffer, F.; Debarbouille, M.; Buschiazzo, A.; Schujman, G.E.; de Mendoza, D.; Alzari, P.M.

2012

Article / Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria

Journal of Biological Chemistry

Urresti, S.; Albesa-Jove, D.; Schaeffer, F.; Pham, H.T.; Kaur, D.; Gest, P.; Van Der Woerd, M.J.; Carreras-González, A.; López-Fernández, S.; Alzari, P.M.; Brennan, P.J.; Jackson, M.; Guerin, M.E.

2011

Article / Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli

Journal of Biological Chemistry

Santangelo, M.D.L.P.; Gest, P.M.; Guerin, M.E.; Coinçon, M.; Pham, H.; Ryan, G.; Puckett, S.E.; Spencer, J.S.; Gonzalez-Juarrero, M.; Daher, R.; Lenaerts, A.J.; Schnappinger, D.; Therisod, M.; Ehrt, S.; Sygusch, J.; Jackson, M.

2010

Article / Rational design, synthesis, and evaluation of new selective inhibitors of microbial class II (zinc dependent) fructose bis-phosphate aldolases

Journal of Medicinal Chemistry

Daher, R.; Coinçon, M.; Fonvielle, M.; Gest, P.M.; Guerin, M.E.; Jackson, M.; Sygusch, J.; Therisod, M.

Short Survey / Molecular Basis of Phosphatidyl-myo-inositol Mannoside Biosynthesis and Regulation in Mycobacteria

Journal of Biological Chemistry

Guerin, M.E.; Korduláková, J.; Alzari, P.M.; Brennan, P.J.; Jackson, M.